Validation of Lucigenin (Bis-N-methylacridinium) as a Chemilumigenic Probe for Detecting Superoxide Anion Radical Production by Enzymatic and Cellular Systems
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چکیده
منابع مشابه
Production of OH-radical-type oxidant by lucigenin.
In the presence of NADH- reductases (dihydrolipoamide: NAD oxidoreductase E. C.1.8.1.4 from pig heart or from Clostridium kluyveri; frequently also addressed as "diaphorases") and NADH lucigenin strongly increases ethylene production from a-keto-methylthiobutyrate (KMB) as an indicator for strong oxidants of the OH-radical type. These reactions are further stimulated in the presence of Fe3+ ion...
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The reduction of ferricytochrome c with adriamycin in phospate buffered saline (pH 7.4) and alkaline solution was obsereved spectrophotometrically. The reduction increased gradually with time of incubation, and decreased with the addition of superoxide dismutase. The semiquinone radical derived from adriamycin was detected by electron spin resonance spectrometer. Superoxide production by analog...
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متن کاملLucigenin chemiluminescence assay for superoxide detection.
Superoxide Detection To the Editor: The recent review by Tarpey and Fridovich published in Circulation Research1 mostly contains interesting and sound material; however, I would like to comment on the section entitled, “Superoxide Detection, Chemiluminescence Reactions.” Basing mainly on their own data,2 authors maintain that lucigenin-amplified chemiluminescence (LucCL) overestimates the rate ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.4.2015